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Discovery of a Novel Amino Lipid That Improves Lipid Nanoparticle Performance through Specific Interactions with mRNA
Author(s) -
Cornebise Mark,
Narayanan Elisabeth,
Xia Yan,
Acosta Edward,
Ci Lei,
Koch Hillary,
Milton Jaclyn,
Sabnis Staci,
Salerno Timothy,
Benenato Kerry E.
Publication year - 2022
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.202106727
Subject(s) - squaramide , messenger rna , coalescence (physics) , nanoparticle , biophysics , nanotechnology , oligonucleotide , materials science , hydrogen bond , in vivo , biochemistry , microbiology and biotechnology , chemistry , biology , molecule , gene , organic chemistry , genetics , catalysis , organocatalysis , astrobiology , enantioselective synthesis
Abstract Lipid nanoparticles (LNPs) are capable of delivering messenger ribonucleic acid (mRNA) efficiently and systemically as a result of both particle composition and architecture. The particle architecture is determined by how the various components interact with one another to establish a stable equilibrium through self‐assembly. The Coulombic attraction between the anionic oligonucleotide cargo and the ionizable amino lipid within the encapsulation media contributes to the coalescence of the particles, but other interactions are also involved. Prior exploration of amino lipids for mRNA delivery via a traditional medicinal chemistry approach suggests that hydrogen bonding between a hydroxyl headgroup and the mRNA contributes to improved LNP in vivo expression. Herein, expansion of that work culminating in the discovery of a novel squaramide lipid that enables robust LNPs with high levels of expression is described. Molecular dynamics simulations and biophysical evidence both support the hypothesis that the squaramide is making specific interactions with mRNA resulting in particles with a more stable and efficient structure.