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Fucoidan Hydrogels Significantly Alleviate Oxidative Stress and Enhance the Endocrine Function of Encapsulated Beta Cells
Author(s) -
Reys Lara L.,
Vaithilingam Vijayaganapathy,
Sthijns Mireille M. J. P. E.,
Soares Eduardo,
Rademakers Timo,
Vries Rick,
Mohammed Sami G.,
Bont Denise,
Jetten Marlon J.,
Hermanns Carolin,
Filho Omar Paulino da Silva,
Stell Adam,
Mourad Nizar I.,
Gianello Pierre,
LaPointe Vanessa L. S.,
Silva Simone S.,
Reis Rui L.,
Silva Tiago H.,
Apeldoorn Aart A.
Publication year - 2021
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.202011205
Subject(s) - fucoidan , oxidative stress , self healing hydrogels , reactive oxygen species , immune system , secretion , inflammation , antioxidant , islet , insulin , microbiology and biotechnology , biology , endocrinology , materials science , biochemistry , polysaccharide , immunology , polymer chemistry
Microencapsulating pancreatic islets in immunoprotective alginate hydrogels is a promising strategy for treatment of type 1 diabetes. However, this strategy is limited by inflammation and hypoxia mediated oxidative stress, due to encapsulation and the hydrogel itself, leading to impaired insulin secretion and limited short and long term cell survival. Herein, the antioxidant effect of fucoidan, an algae derived polysaccharide, on beta cells, and its positive effects on encapsulated beta cell viability and function is presented. Fucoidan from Fucus vesiculosus (FF) exhibits a high total antioxidant capacity, and free radical scavenging activity, and is able to significantly alleviate intracellular oxidative stress in rat insolinoma beta cells (INS1E). In addition, FF significantly increases insulin secretion in a dose‐ and time‐dependent manner. When FF is incorporated in ultrapure alginate used for microencapsulation of primary rat islets, both viability and glucose responsiveness of rat islets in these socalled Fucogel microcapsules (Fucocaps) are found to be significantly higher compared to islets encapsulated in alginate alone. Similar results are obtained with INS1E pseudoislets and neonatal pig islets. Fucocaps can provide a redox‐modulatory niche and an immune barrier for islets and beta cells in the same time leading to significantly improved survival and endocrine function by mitigating oxidative stress.