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Association between Cell Microenvironment Altered by Gold Nanowire Array and Regulation of Partial Epithelial‐Mesenchymal Transition
Author(s) -
Kim Yu Jin,
Ko Min Jun,
Park Bum Chul,
Moon Jun Hwan,
Jeon Yoo Sang,
Park Hyeon Su,
Kim Myeong Soo,
Kim HeeDae,
Shin Daiha,
Kim Young Keun
Publication year - 2021
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.202008758
Subject(s) - mesenchymal stem cell , microbiology and biotechnology , cell adhesion , epithelial–mesenchymal transition , cell , adhesion , cell adhesion molecule , downregulation and upregulation , intracellular , focal adhesion , chemistry , materials science , biology , signal transduction , biochemistry , gene , organic chemistry
Cell microenvironment is an essential factor in determining cell growth and cell fate. Many studies have been carried out to understand the functions and mechanisms of small molecules or growth factors/cytokines; however, the effects of the physical environment on cells are relatively unknown. Changes in the cell's physical microenvironment affect cell adhesion and modify intracellular signaling controlled by adhesion properties, resulting in altering the cytoskeletal structure and cellular properties. Herein, it is demonstrated that the changes in cell adhesion can affect the epithelial‐mesenchymal transition (EMT) of epithelial cells by implementing a cell microenvironment with a gold (Au) nanowire array to influence cell adhesion. A forcible decrease in cell adhesion leads to the downregulation of epithelial biomarkers and the upregulation of mesenchymal biomarkers. The results of force‐distance experiments using atomic force microscopy showed that the overall stiffness of epithelial cells declined similarly to the case for mesenchymal‐like cells. With this comprehensive analysis of cellular properties, a physical microenvironment for cell adhesion alteration is suggested, that can induce mesenchymal characteristics in both epithelial and mesenchymal cells through partial EMT.