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A Collagen Based Cryogel Bioscaffold that Generates Oxygen for Islet Transplantation
Author(s) -
Razavi Mehdi,
Primavera Rosita,
Kevadiya Bhavesh D.,
Wang Jing,
Buchwald Peter,
Thakor Avnesh S.
Publication year - 2020
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.201902463
Subject(s) - islet , transplantation , biocompatibility , pancreatic islets , biomedical engineering , biocompatible material , tissue engineering , insulin , materials science , andrology , chemistry , endocrinology , medicine , metallurgy
The aim of this article is to develop, characterize, and test a novel 3D bioscaffold matrix that can accommodate pancreatic islets and provide them with a continuous, controlled, and steady source of oxygen to prevent hypoxia‐induced damage following transplantation. Hence, a collagen‐based cryogel bioscaffold that incorporates calcium peroxide (CPO) into its matrix is made. The optimal concentration of CPO integrated into bioscaffolds is 0.25 wt% and this generates oxygen at 0.21 ± 0.02 × 10 ‐3 m day ‐1 (day 1), 0.19 ± 0.01 × 10 ‐3 m day ‐1 (day 6), 0.13 ± 0.03 × 10 ‐3 m d ‐1 (day 14), and 0.14 ± 0.02 × 10 ‐3 m d ‐1 (day 21). Accordingly, islets seeded into cryogel‐CPO bioscaffolds have a significantly higher viability and function compared to islets seeded into cryogel alone bioscaffolds; these findings are supported by data from quantitative computational modeling. When syngeneic islets are transplanted into the epididymal fat pad (EFP) of diabetic mice, the cryogel‐0.25 wt%CPO bioscaffold improves islet function with diabetic animals re‐establishing glycemic control. Mice transplanted with cryogel‐0.25 wt%CPO bioscaffolds show faster responses to intraperitoneal glucose injections and have a higher level of insulin content in their EFP compared to those transplanted with islets alone ( P < 0.05). The novel oxygen‐generating bioscaffold (i.e., cryogel‐0.25 wt%CPO) therefore provides a biostable and biocompatible 3D microenvironment for islets which can facilitate islet survival and function at extra‐hepatic sites of transplantation.