Premium
Ribonucleoprotein: A Biomimetic Platform for Targeted siRNA Delivery
Author(s) -
Tai Wanyi,
Gao Xiaohu
Publication year - 2019
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.201902221
Subject(s) - gene knockdown , ribonucleoprotein , small interfering rna , rna , nanotechnology , rna interference , materials science , biophysics , translation (biology) , in vitro , cationic polymerization , biology , messenger rna , biochemistry , gene , polymer chemistry
Many small interfering RNA (siRNA) carriers have been developed over the years, mostly based on cationic lipids and polymers that can condense siRNA into nanoparticle complexes and aid in endosomal escape. In comparison, development of charge‐neutral siRNA carriers to avoid or reduce nonspecific binding, aggregation, and toxicity is limited, due to a lack of mechanisms for carrier‐siRNA association beyond electrostatic interaction. Here, a unique, charge‐neutral, biomimetic platform is reported, mimicking the natural state of functional RNA, ribonucleoprotein (RNP). This RNP architecture is simple to make, precise in assembly stoichiometry, stable in serum, and biocompatible. Gene knockdown is achieved in vitro and in vivo, demonstrating excellent potential for translation.