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Functionalized Bioink with Optical Sensor Nanoparticles for O 2 Imaging in 3D‐Bioprinted Constructs
Author(s) -
Trampe Erik,
Koren Klaus,
Akkineni Ashwini Rahul,
Senwitz Christian,
Krujatz Felix,
Lode Anja,
Gelinsky Michael,
Kühl Michael
Publication year - 2018
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.201804411
Subject(s) - materials science , 3d bioprinting , nanotechnology , nanoparticle , viability assay , biocompatible material , 3d cell culture , biophysics , cell , biomedical engineering , tissue engineering , chemistry , biochemistry , medicine , biology
Research on 3D bioprinting of living cells has strong focus on printable biocompatible materials and monitoring of cell growth in printed constructs, while cell metabolism is mostly measured in media surrounding the constructs or by destructive sample analyses. Bioprinting is combined with online imaging of O 2 by functionalizing a hydrogel bioink via addition of luminescent optical sensor nanoparticles. Rheological properties of the bioink enable 3D printing of hydrogel layers with uniform response to O 2 concentration. Co‐immobilization of sensor nanoparticles with green microalgae and/or mesenchymal stem cells does not affect cell viability over several days. Interference from microalgal autofluorescence on the O 2 imaging is negligible, and no leakage or photobleaching of nanoparticles is observed over 2–3 days. Oxygen dynamics due to respiration and photosynthesis of cells can be imaged online and the metabolic activity of different cell types can be discriminated in intact 3D structures. Bioinks containing chemical sensor particles enable noninvasive mapping of cell metabolism and spatiotemporal dynamics of their chemical microenvironment in 3D‐printed structures. This major advance now facilitates rapid evaluation of cell activity in printed constructs as a function of structural complexity, metabolic interactions in mixed species bioprints, and in response to external incubation conditions.