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Allochroic‐Graphene Oxide Linked 3D Oriented Surface Imprinting Strategy for Glycoproteins Assays
Author(s) -
Chen Guosheng,
Kou Xiaoxue,
Huang Siming,
Huang Shuyao,
Zhang Rui,
Liu Chao,
Shen Jun,
Zhu Fang,
Ouyang Gangfeng
Publication year - 2018
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.201804129
Subject(s) - graphene , nanotechnology , materials science , oxide , molecular imprinting , imprinting (psychology) , surface modification , point of care , glycoprotein , computer science , chemistry , biochemistry , medicine , selectivity , nursing , metallurgy , gene , catalysis
Affordable methodologies for screening of disease‐related biomarkers are vital for early clinical diagnosis, and can potentially improve the survival rate. Herein, a cost‐efficient and easy‐to‐use approach, called allochroic‐graphene oxide linked oriented surface imprinting strategy (AGO‐OSI), is imparted for rapid glycoproteins screening with naked eyes. The novel detection principle herein relies on the boronate‐affinity sandwich‐like assembly, in which the target glycoproteins are specifically captured by the engineered 3D oriented surface imprinting magnetic microprobe, and followed by labeling with the pH‐triggered allochroic‐graphene oxide. Compared with the enzyme‐linked immunosorbent assay, no antibody‐assisted recognition or complicated enzymatic labeling is required, endowing the AGO‐OSI with the merits of low‐cost, labor‐saving, and user‐friendly. In addition, the pH‐triggered allochroic‐graphene oxide provides a simple, real‐time, and economical signal readout, without the sophisticated instruments requirement. Finally, it is successfully applied to rapid diagnosis of α‐fetoprotein (AFP) in liver cancer event with naked eyes, further manifesting the superiority of AGO‐OSI. It is believed that the cost‐efficient and easy‐to‐use strategy proposed herein can potentially be a versatile tool for point‐of‐care diagnosis, especially applicable in resource‐constrained regions.