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Living Bacteria in Thermoresponsive Gel for Treating Fungal Infections
Author(s) -
Lufton Maayan,
Bustan Or,
Eylon Bathen,
ShtifmanSegal Ella,
CroitoruSadger Tsuf,
Shagan Alona,
ShabtayOrbach Ayelet,
CoremSalkmon Enav,
Berman Judith,
Nyska Abraham,
Mizrahi Boaz
Publication year - 2018
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.201801581
Subject(s) - stratum corneum , bacteria , materials science , rational design , bacillus subtilis , dermis , microbiology and biotechnology , ketoconazole , drug delivery , in vivo , antifungal , nanotechnology , biology , genetics , anatomy
The leading living bacteria formulations currently available are from a limited list of genera and are generally limited to gastrointestinal tract syndromes. A formulation composed of living Bacillus subtilis incorporated in a thermoresponsive hydrogel that hardens after administration on the skin and continuously produces antifungal agents is described. The ability of the formula to support bacteria growth and its mechanical properties and penetrability through the skin are fine‐tuned by varying the ratio between polymer concentrations and bacterial media. The formula penetrates via the stratum corneum and accumulates in the epidermis without penetrating the inner, dermis layer. In vivo results mirror the results seen in vitro: bacillus formulations completely inhibit candida growth, demonstrating clinical effects comparable to those achieved by ketoconazole. LC‐MS/MS analysis of the bacterial formulation confirms the presence of surfactin, the most powerful biosurfactant that possesses a broad antifungal activity. This platform may enable rational design of novel formulations composed of secreting bacteria inside a responsive, smart, hydrogel—which is the prerequisite for producing a successful drug delivery system.