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Photogenerated Aldehydes for Protein Patterns on Hydrogels and Guidance of Cell Behavior
Author(s) -
Ming Zunzhen,
Fan Jinyan,
Bao Chunyan,
Xue Yuan,
Lin Qiuning,
Zhu Linyong
Publication year - 2018
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.201706918
Subject(s) - self healing hydrogels , aldehyde , materials science , extracellular matrix , imine , covalent bond , nanotechnology , cell , tissue engineering , biophysics , combinatorial chemistry , chemistry , biochemistry , biology , organic chemistry , polymer chemistry , genetics , catalysis
The immobilization of proteins to hydrogels is important and plays a significant role to provide suitable biomimetic material as extracellular matrix for cell behavior mediation. This study describes a novel and universal strategy for photopatterning unmodified proteins on hydrogels. The methodology creates photogenerated aldehyde regions within a protein‐resistant hydrogel and then conjugates unmodified proteins by mild imine ligation with spatial, temporal, and dosage control. The relatively stable aldehyde intermediate enables the facile and highly efficient covalent immobilization of proteins by a postfunctionalization methodology and the sequential protein patterns provide an easy access to control the identity and dynamic change of proteins presented to cells on demand, thus mediating cell behaviors. This approach provides important opportunities for understanding and controlling cell behavior mediated by proteins, and opens up new avenues for hydrogels in tissue engineering and biotechnology applications.