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A Multicolor Chameleon DNA‐templated Silver Nanocluster and Its Application for Ratiometric Fluorescence Target Detection with Exponential Signal Response
Author(s) -
Zhou Weijun,
Zhu Jinbo,
Fan Daoqing,
Teng Ye,
Zhu Xiaoqing,
Dong Shaojun
Publication year - 2017
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.201704092
Subject(s) - fluorescence , materials science , signal (programming language) , dna , optics , optoelectronics , analytical chemistry (journal) , physics , chemistry , computer science , biochemistry , chromatography , programming language
Abstract For the first time, a novel type of chameleon DNA‐templated silver nanocluster (AgNC) is found whose fluorescence color can be switched among yellow, orange, and red by the regulation of complementary DNA, nonfluorescent assistant AgNC as well as Mg 2+ . AgNC templated by A 20 ‐C55 (A 20 ‐C55‐NC) possesses strong yellow fluorescence (Y signal) in phosphate buffer solution. When approaching to the nonfluorescent assistant AgNC through template hybridization, Y signal decreases while a new red emission (R signal) rises, leading to a dramatic color change of AgNC solution from yellow to red. On the other hand, hybridization of A 20 ‐C55‐NC with complementary DNA (T 20 ) largely enhances the Y signal while A 20 ‐C55‐NC shows R and Y signal with equal intensity simultaneously in the presence of Mg 2+ . Therefore, the chameleon AgNC achieves controllable multicolor fluorescence variation. Based on above mechanism, a series of ratiometric analysis platforms are constructed for DNA target detection. Surprisingly, the ratiometric probes demonstrate an exponential growth of signal response with nanomolar sensitivity whether in double‐stranded or hairpin‐shaped structure. Accordingly, this universal ratiometric analysis platform possesses low background, large signal variation in a narrow concentration range, which presents obvious advantages over most of previous DNA detection strategies that are based on DNA‐templated AgNC.