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Micropatterned Protein for Cell Adhesion through Phototriggered Charge Change in a Polyvinylpyrrolidone Hydrogel
Author(s) -
Ming Zunzhen,
Ruan Xing,
Bao Chunyan,
Lin Qiuning,
Yang Yi,
Zhu Linyong
Publication year - 2017
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.201606258
Subject(s) - self healing hydrogels , zwitterion , materials science , polyvinylpyrrolidone , protein adsorption , adhesion , adhesive , cell adhesion , nanotechnology , bioadhesive , polymer , biophysics , chemical engineering , drug delivery , polymer chemistry , chemistry , organic chemistry , layer (electronics) , molecule , engineering , composite material , biology
Regulated immobilization of proteins on hydrogels allows for the creation of highly controlled microenvironments to meet the special requirements of cell biology and tissue engineering devices. Light is an ideal stimulus to regulate immobilization because it can be controlled in time, space, and intensity. Here, a photoresponsive hydrogel that enables the patterning of proteins by a combination of electrostatic adsorption and photoregulated charge change on a hydrogel is developed. It is based on a photosensitive cationic monomer ( CLA ), a coumarin caged lysine betaine zwitterion, incorporated into a polyvinylpyrrolidone ( PVP ) hydrogel, which can controllably change the charge from an adhesive positive state to an anti‐adhesive zwitterion state upon irradiation at 365 nm. With this strategy, the immobilization of proteins is regulated and cell adhesion is programmed on hydrogels on demand. This approach should open up new avenues for hydrogels in biomedical applications.