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A Protein‐Nanocellulose Paper for Sensing Copper Ions at the Nano‐ to Micromolar Level
Author(s) -
Weishaupt Ramon,
Siqueira Gilberto,
Schubert Mark,
Kämpf Michael M.,
Zimmermann Tanja,
ManiuraWeber Katharina,
Faccio Greta
Publication year - 2017
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.201604291
Subject(s) - nanocellulose , materials science , biosensor , copper , fluorometer , biomolecule , fluorescence , nanotechnology , detection limit , nanosensor , chemical engineering , chromatography , chemistry , optics , cellulose , physics , engineering , metallurgy
Tracing heavy metals is a crucial issue in both environmental and medical samples. In this work, a sensing biomolecule, the cyanobacterial C‐phycocyanin (CPC), is integrated into a nanocellulose matrix, and with this, a biosensor for copper ions is developed. The assembly of CPC‐functionalized nanocellulose into a red‐fluorescent, copper‐sensitive hybrid film “CySense”, enhances protein stability and facilitates the reuse and the regeneration of the sensor for several cycles over 7 days. CySense is suitable for the analysis of complex medical samples such as human serum filtrate. The reported biosensor reliably detects copper ion contents with a lower detection limit of 200 × 10 −9 m and an IC50 of 4.9 × 10 −6 m as changes in fluorescence emission intensity that can be measured with a fluorimeter or a microarray laser scanner.