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Scissor‐Like Chiral Metamolecules for Probing Intracellular Telomerase Activity
Author(s) -
Sun Maozhong,
Xu Liguang,
Fu Pan,
Wu Xiaoling,
Kuang Hua,
Liu Liqiang,
Xu Chuanlai
Publication year - 2016
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.201601942
Subject(s) - intracellular , materials science , telomerase , circular dichroism , chirality (physics) , biophysics , hela , colloidal gold , cancer cell , nanotechnology , nanoparticle , cell , chemistry , biochemistry , cancer , biology , genetics , chiral symmetry breaking , physics , quantum mechanics , nambu–jona lasinio model , gene , quark
A DNA‐driven gold (Au) heterodimer for intracellular telomerase detection is fabricated. The highly biocompatible and intracellularly stable probe shows an active chiroptical property in the visible region, due to the scissor‐like configuration formed by prolate nanoparticles. Importantly, the telomerase activity is specifically quantified using circular dichroism intensity in situ after internalization of the heterodimer into cancer cells. Moreover, the results clearly illustrate that this method has a remarkable linear range from 0.8 × 10 −12 to 32 × 10 −12 IU, and the limit of detection for telomerase activity is 1.7 × 10 −15 IU in a single HeLa cell. This strategy paves the way for chirality‐based ultrasensitive detection of intracellular cancer markers.
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