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Graphene‐Oxide‐Conjugated Polymer Hybrid Materials for Calmodulin Sensing by Using FRET Strategy
Author(s) -
Yuan Hongbo,
Qi Junjie,
Xing Chengfen,
An Hailong,
Niu Ruimin,
Zhan Yong,
Fan Yibing,
Yan Wenmin,
Li Ruihua,
Wang Bing,
Wang Shu
Publication year - 2015
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.201501668
Subject(s) - graphene , calmodulin , förster resonance energy transfer , materials science , conjugated system , oxide , polymer , biomolecule , fluorescence , nanotechnology , biophysics , combinatorial chemistry , chemistry , calcium , physics , metallurgy , composite material , quantum mechanics , biology
The conformation of calmodulin (CaM) changes from closed configuration to open one, converting to a claviform dumbbell‐shaped biomolecule upon Ca 2+ ‐binding. A hybrid probe of graphene oxide (GO) cationic conjugated polymer for detection of the conformation transition of CaM by using FRET technique is demonstrated. The stronger hydrophobic interaction and weaker electrostatic repulsion leads to more CaM adsorption to the surface of GO upon binding with Ca 2+ than that of CaM in the absence of Ca 2+ (apoCaM), resulting in much farther proximity between poly[(9,9‐bis(6′‐ N , N , N ‐trimethylammonium)hexyl)‐fluorenylene phenylene dibromide] (PFP) and green fluorescent protein labeled at the N‐terminus of CaM and therefore much weaker FRET efficiency for PFP/Ca 2+ /CaM in comparison with that of PFP/apoCaM in the presence of GO. Notably, the assembly of CaM with GO is quantitatively and reversibly controlled by Ca 2+ ions.