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Electrochemical Quantification of Escherichia coli with DNA Nanostructure
Author(s) -
Giovanni Marcella,
Setyawati Magdiel Inggrid,
Tay Chor Yong,
Qian Hang,
Kuan Win Sen,
Leong David Tai
Publication year - 2015
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.201500940
Subject(s) - escherichia coli , lysis , bacteria , dna , microbiology and biotechnology , contamination , materials science , biology , biochemistry , gene , genetics , ecology
Escherichia coli is an indicator of fecal contamination in recreational water and some strains are responsible for serious food poisoning in humans. Therefore, having a rapid, sensitive, selective, and simple E. coli detection is important. In this study, DNA nanopyramids anchor is utilized for immunological detection of E. coli lipopolysaccharides, lysate, and whole bacteria. By measuring the E. coli amount electrochemically, detection limits of 0.20 ng mL −1 E. coli lipopolysaccharides and 1.20 CFU mL −1 equivalent of lysed E. coli bacteria are obtained. Our method confers selectivity to detect E. coli in the presence of various other macromolecules. Therefore, our system can be developed to test the E. coli content in real water samples.