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Materials Displaying Neural Growth Factor Gradients and Applications in Neural Differentiation of Embryoid Body Cells
Author(s) -
Delalat Bahman,
Mierczynska Agnieszka,
Ghaemi Soraya Rasi,
Cavallaro Alex,
Harding Frances J.,
Vasilev Krasimir,
Voelcker Nicolas H.
Publication year - 2015
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.201500595
Subject(s) - embryoid body , materials science , biophysics , reductive amination , nerve growth factor , embryonic stem cell , chemistry , biochemistry , biology , receptor , adult stem cell , gene , catalysis
The critical growth factor density required to support neural lineage generation from mouse embryonic stem cells is assessed by constructing a surface density gradient of immobilized nerve growth factor (NGF) from a plasma polymer film base. A chemical surface gradient varying from high hydroxyl group density to high aldehyde group density is prepared through diffusion‐controlled plasma polymerization of two monomers (ethanol and propionaldehyde) under a moving mask. NGF density gradients are then produced by reductive amination with the aldehyde groups on the plasma polymer surface. Mouse embryoid body derived (mEB) cell differentiation on the gradient surface is evaluated by immunofluorescence staining against Nestin. mEB cell density and the percentage of Nestin‐positive cells increase with increasing NGF density up to a critical value corresponding to 52.9 ng cm −2 , above which cell attachment and differentiation do not increase further. This gradient‐based screening approach allows the growth factor surface densities to be optimized for biomaterials intended for cell differentiation or expansion, which is highly relevant to creating efficient manufacture processes for cell therapies.