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2D and 3D Hybrid Systems for Enhancement of Chondrogenic Differentiation of Tonsil‐Derived Mesenchymal Stem Cells
Author(s) -
Park Jinhye,
Kim In Young,
Patel Madhumita,
Moon Hyo Jung,
Hwang SeongJu,
Jeong Byeongmoon
Publication year - 2015
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.201500299
Subject(s) - materials science , chondrogenesis , peg ratio , mesenchymal stem cell , ethylene glycol , 3d cell culture , hybrid system , cell culture , nanotechnology , graphene , stem cell , chemical engineering , biophysics , cell , microbiology and biotechnology , chemistry , biochemistry , biology , computer science , genetics , finance , machine learning , engineering , economics
2D/3D hybrid cell culture systems are constructed by increasing the temperature of the thermogelling poly(ethylene glycol)‐poly( l ‐alanine) diblock copolymer (PEG‐ l ‐PA) aqueous solution in which tonsil tissue‐derived mesenchymal stem cells and graphene oxide (GO) or reduced graphene oxide (rGO) are suspended, to 37 °C. The cells exhibit spherical cell morphologies in 2D/3D hybrid culture systems of GO/PEG‐ l ‐PA and rGO/PEG‐ l ‐PA by using the growth medium. The cell proliferations are 30%–50% higher in the rGO/PEG‐ l ‐PA hybrid system than in the GO/PEG‐ l ‐PA hybrid system. When chondrogenic culture media enriched with TGF‐β3 is used in the 2D/3D hybrid systems, cells extensively aggregate, and the expression of chondrogenic biomarkers of SOX 9, COL II A1, COL II, and COL X significantly increases in the GO/PEG‐ l ‐PA 2D/3D hybrid system as compared with the PEG‐ l ‐PA 3D systems and rGO/PEG‐ l ‐PA 2D/3D hybrid system, suggesting that the GO/PEG‐ l ‐PA 2D/3D hybrid system can be an excellent candidate as a chondrogenic differentiation platform of the stem cell. This paper also suggests that a 2D/3D hybrid system prepared by incorporating 2D materials with various surface biofunctionalities in the in situ forming 3D hydrogel matrix can be a new cell culture system.