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The Effect of Protein Fusions on the Production and Mechanical Properties of Protein‐Based Materials
Author(s) -
Tsai ShangPu,
Howell David W.,
Huang Zhao,
Hsiao HaoChing,
Lu Yang,
Matthews Kathleen S.,
Lou Jun,
Bondos Sarah E.
Publication year - 2015
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.201402997
Subject(s) - biomolecule , fusion protein , materials science , solubility , protein engineering , biophysics , nanotechnology , biochemistry , recombinant dna , gene , chemistry , biology , organic chemistry , enzyme
Proteins implement most of the vital molecular functions of living organisms, including structural support, energy generation, biomolecule sensing, and chemical catalysis, storage, and degradation. While capturing proteins in materials could create devices that mimic these functions, this process is challenging due to the sensitivity of protein structure to the chemical environment. Using recombinant DNA methods, specific functions can be incorporated by fusing the gene encoding a self‐assembling protein and the desired functional protein, to produce a single polypeptide that self‐assembles into functionalized materials. However, the functional protein has the potential to disrupt protein production, protein assembly, and/or the structure and mechanical properties of the resulting materials. 24 fusion proteins are created based on Ultrabithorax, a Drosophila transcription factor that self‐assembles into materials in vitro. The appended proteins dictate the solubility and purification yield of the corresponding protein fusions. Any loss of solubility and yield can be mitigated by fusing a third protein that is highly soluble. All protein fusions self‐assemble equally well to produce materials with similar morphologies. Fusing enhanced green fluorescent protein to Ultrabithorax influences mechanical properties of the resulting fibers. It is concluded that a far wider range of proteins can be successfully incorporated into elastomeric protein‐based materials than originally anticipated.