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Bio‐Inspired Preparation of Fibrin‐Boned Bionanocomposites of Biomacromolecules and Nanomaterials for Biosensing
Author(s) -
Han Fangfang,
Qi Xin,
Li Lingyan,
Bu Lijuan,
Fu Yingchun,
Xie Qingji,
Guo Manli,
Li Yanbin,
Ying Yibin,
Yao Shouzhuo
Publication year - 2014
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.201400458
Subject(s) - materials science , nanomaterials , biosensor , nanotechnology , biomimetics , biomimetic materials , chemical engineering , engineering
Learning from nature is one of the most promising ways to develop advanced functional materials. Here, inspired by blood coagulation, novel fibrin‐boned bionanocomposites are reported as efficient immobilization matrices of biomacromolecules and nanomaterials for biosensing. Glucose oxidase (GOx), Au nanoparticles (AuNPs), and Fe 3 O 4 magnetic nanoparticles (MNPs) are adopted as the model biomacromolecules and nanomaterials. By integrating the thrombin‐triggered coagulation of fibrin with advanced surficial modification techniques, four kinds of immobilization strategies are developed and evaluated. Digital imaging, UV‐vis spectroscopy, scanning/transmission electron microscopy, electrochemical methods, and N 2 adsorption‐desorption isotherms are used to investigate the formation, immobilization efficiency, and performance of various bionanocomposites. The fibrin‐boned networks show inherent biocompatibility, excellent adsorbability, porosity, and functionalization ability, endowing the bionanocomposites with high efficiencies in capturing AuNPs, MNPs and GOx (99%, 98%, and 57% captured under the given conditions, respectively), as well as significant mass‐transfer and biocatalysis efficiencies. Therefore, the fibrin‐boned bionanocomposites show great potential for biosensing, for example, a fibrin‐AuNPs‐GOx‐glutaraldehyde bionanocomposites modified Au electrode is highly sensitive to glucose (145 μA cm −2 mM −1 ) allowing for a limit of detection down to 25 nM, being much superior to those of the reported analogues. The presented experimental platform/strategy may find wide applications in the development of other bio/nano‐materials/devices.