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Hierachically Structured Hollow Silica Spheres for High Efficiency Immobilization of Enzymes
Author(s) -
Cao Shunsheng,
Fang Long,
Zhao Zhiyuan,
Ge Yi,
Piletsky Sergey,
Turner Anthony P. F.
Publication year - 2013
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.201201793
Subject(s) - materials science , glucose oxidase , immobilized enzyme , leaching (pedology) , adsorption , chemical engineering , biocatalysis , spheres , biosensor , nanotechnology , shell (structure) , enzyme , catalysis , organic chemistry , composite material , chemistry , reaction mechanism , environmental science , physics , astronomy , soil science , engineering , soil water
In this work, the first example of a hierarchically structured hollow silica system is reported without any chemical modification to the enzyme involved in the process. The leaching of the physically adsorbed enzyme is substantially restrained in comparison to pure hollow silica supports. The hierarchical architecture is composed of the ordered hollow silica spheres with a shell‐in‐shell structure. This rationally integrated architecture, which serves as the host for glucose oxidase immobilization, displays many significant advantages, including increases in mechanical stability, enzyme loading, and bioactivity, and a decrease in enzyme leaching compared to existing pure hollow silica matrices. This facilitates further multifarious applications for enhanced enzyme immobilization, biosensors, and biocatalysis.