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Blood‐Inert Surfaces via Ion‐Pair Anchoring of Zwitterionic Copolymer Brushes in Human Whole Blood
Author(s) -
Chang Yung,
Shih YuJu,
Lai ChiaJung,
Kung HsiaoHan,
Jiang Shaoyi
Publication year - 2013
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.201201386
Subject(s) - copolymer , atom transfer radical polymerization , materials science , methacrylate , polymer chemistry , polymer , surface modification , protein adsorption , chemical engineering , composite material , engineering
A strategy to create blood‐inert surfaces in human whole blood via ion‐pair anchoring of zwitterionic copolymer brushesand a systematic study of how well‐defined chain lengths and well‐controlled surface packing densities of zwitterionic polymers affect blood compatibility are reported. Well‐defined diblock copolymers, poly(11‐mercaptoundecyl sulfonic acid)‐ block ‐poly(sulfobetaine methacrylate) (PSA‐ b ‐PSBMA) with varying zwitterionic PSBMA or negatively charged PSA lengths, are synthesized via atom‐transfer radical polymerization (ATRP). PSA‐ b ‐PSBMA is grafted onto a surface covered with polycation brushes as a mimic polar/hydrophilic biomaterial surface via ion‐pair anchoring at a range of copolymer concentrations. Protein adsorption from single‐protein solutions, 100% blood serum, and 100% blood plasma onto the surfaces covered with PSA‐ b ‐PSBMA brushes is evaluated using a surface plasmon resonance sensor. Copolymer brushes containing a high amount of zwitterionic SBMA units are further challenged with human whole blood. Low protein‐fouling surfaces with >90% reduction with respect to uncoated surfaces are achieved with longer PSA blocks and higher concentrations of PSA‐ b ‐PSBMA copolymers using the ion‐pair anchoring approach. This work provides a platform to achieve the control of various surface parameters and a practical method to create blood‐inert surfaces in whole blood by grafting ionic‐zwitterionic copolymers to charged biomaterials via charge pairing.