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Screening Mesenchymal Stem Cell Attachment and Differentiation on Porous Silicon Gradients
Author(s) -
Wang PengYuan,
Clements Lauren R.,
Thissen Helmut,
Jane Andrew,
Tsai WeiBor,
Voelcker Nicolas H.
Publication year - 2012
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.201200447
Subject(s) - materials science , mesenchymal stem cell , ridge , nanotechnology , surface roughness , nanometre , tissue engineering , adhesion , surface finish , nanotopography , cell adhesion , regenerative medicine , nanoscopic scale , anodizing , porosity , stem cell , biomedical engineering , composite material , microbiology and biotechnology , aluminium , biology , medicine , paleontology
The profound effects that nanoscale surface topography exerts on cell behavior are highly relevant to the development of advanced biomaterials and to advances in tissue engineering and regenerative medicine. Here, an asymmetric anodization procedure is used to produce n‐type porous silicon (pSi) gradients with pore sizes ranging from tens to hundreds of nanometers in diameter and changes in the ridge nanoroughness from a few to tens nanometers. Rat mesenchymal stem cells (rMSCs) adhere poorly at the regions with small pore size but high ridge roughness. Cell adhesion is increased gradually towards the large pore size but low ridge roughness end of the pSi gradients. Surface topography influences cell differentiation, but not cell proliferation. Osteogenesis of rMSCs is enhanced by porous topography with a ridge roughness lower than 10 nm, while adipogenesis of rMSCs is enhanced on the entire pSi gradient compared with flat Si substrates. The results demonstrate that the gradient format allows in‐depth screening of surface parameters that are important for the control of mammalian cell behavior, thereby advancing the development of new and improved biomaterials for orthopaedic and tissue engineering applications.