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Water‐Soluble Conjugated Polymers for Amplified Fluorescence Detection of Template‐Independent DNA Elongation Catalyzed by Polymerase
Author(s) -
He Fang,
Liu Libing,
Li Lidong
Publication year - 2011
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.201100216
Subject(s) - polyfluorene , fluorescence , polymer , intramolecular force , materials science , fluorene , aqueous solution , conjugated system , elongation , terminal deoxynucleotidyl transferase , copolymer , förster resonance energy transfer , dna , derivative (finance) , photochemistry , polymer chemistry , organic chemistry , chemistry , biochemistry , apoptosis , physics , quantum mechanics , tunel assay , metallurgy , composite material , ultimate tensile strength , economics , financial economics
A series of water‐soluble polyfluorene derivatives containing diketopyrrolopyrrole derivative units are synthesized and characterized. These copolymers, poly[9,9'‐bis(6”‐ N , N , N ‐trimethyl ammonium) hexylfluorene‐ co ‐ alt ‐2,5‐bis (6”‐ N , N , N ‐trimethylammonium)hexylpyrrolo[3,4‐c]pyrrole‐1,4(2H,5H)‐dione] (PFDPN), demonstrate intramolecular energy transfer from the fluorene units to the diketopyrrolopyrrole derivative units, and show red‐shifted emissions in aqueous solution. The PFDPN polymers can combine with Cy5‐labeled ssDNA by electrostatic interactions and efficiently amplify the fluorescence signal of red Cy5 dye through fluorescence resonance energy transfer. Moreover, based on DNA replacement method, this amplification system can be used to monitor the template‐independent DNA elongation process catalyzed by terminal deoxynucleotidyl transferase.