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Biocompatible Bright YVO 4 :Eu Nanoparticles as Versatile Optical Bioprobes
Author(s) -
Shen Jie,
Sun LingDong,
Zhu JiaDan,
Wei LiuHe,
Sun HongFang,
Yan ChunHua
Publication year - 2010
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.201090098
Subject(s) - materials science , biocompatibility , nanoparticle , luminescence , fluorescence , polyacrylic acid , nanomaterials , circular dichroism , conjugated system , lanthanide , nanotechnology , nuclear chemistry , chemical engineering , polymer , chemistry , organic chemistry , crystallography , ion , physics , optoelectronics , quantum mechanics , engineering , metallurgy , composite material
Lanthanide nanoparticles (NPs), characterized by their large Stokes’ shifts, narrow emission bands and high photochemical stability, are promising candidates for optical probes in biological research. As a typical red emission material, Eu 3+ ‐doped YVO 4 is investigated in pursuit of an integral methodology for Ln‐based bioprobes. Assisted with phosphino‐polyacrylic acid, YVO 4 :Eu NPs with a luminescent quantum yield of ca . 54% are synthesized via a one‐pot hydrothermal reaction. The obtained NPs exhibit good water/buffer stability and feasible biomodification benefiting from the surface carboxylic acid groups. However, having an inorganic core of ca. 20 nm, these NPs do not affect the conformation of surface conjugated proteins (bovine serum albumin), as confirmed with the circular dichroism spectrum. A specific recognition protocol is demonstrated by biotinated YVO 4 :Eu NPs in quantitative fluoroimmunoassay and microchip assay. Furthermore, the biocompatibility of the Ln NPs, which is an important aspect towards biological application, is demonstrated with the MTT [3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide] assay. Cell imaging studies conducted with spectral mapping technique and confocal fluorescent microscopy demonstrate the YVO 4 :Eu NPs to be a type of competitive luminescent probes for in vitro applications.