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Pegylated Composite Nanoparticles Containing Upconverting Phosphors and meso ‐Tetraphenyl porphine (TPP) for Photodynamic Therapy
Author(s) -
Shan Jingning,
Budijono Stephanie J.,
Hu Guohong,
Yao Nan,
Kang Yibin,
Ju Yiguang,
Prud'homme Robert K.
Publication year - 2011
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.201002516
Subject(s) - photodynamic therapy , materials science , nanoparticle , ethylene glycol , photosensitizer , hela , composite number , peg ratio , nanotechnology , photochemistry , in vitro , chemistry , organic chemistry , composite material , biochemistry , finance , economics
The utilization of upconverting nanophosphors (UCNP) for photodynamic therapy (PDT) has gained significant interests due to its ability to convert deep‐penetrating near‐infra red (NIR) light (i.e., 978 nm) to visible light. Previous attempts to co‐localize UCNPs with photosensitizers suffer from low photosensitizer loading and problems with nanoparticle aggregation. Here, the preparation of a novel composite nanoparticle formulation comprising 100 nm β −NaYF 4 :Yb 3+ ,Er 3+ UCNPs, and meso ‐tetraphenyl porphine (TPP) photosensitizer, stabilized by biocompatible poly(ethylene glycol‐ block ‐( dl )lactic acid) block copolymers (PEG‐ b ‐PLA) is presented. A photosensitizer loading of 10 wt% with respect to UCNP crystal was achieved via the Flash NanoPrecipitation (FNP) process. A sterically stabilizing PEG layer on the composite nanoparticle surface prevents nanoparticle aggregation and ensures nanoparticle stability in water, PBS buffer, and culture medium containing serum proteins, resulting in nanoparticle suitable for in vivo applications. Based on in vitro studies utilizing HeLa cervical cancer cell lines, the composite nanoparticles are shown to exhibit low dark toxicity and efficient cancer cell‐killing activity upon NIR excitation. Exposure with 134 W cm −2 of 978 nm light for 45 min resulted in 75% HeLa cell death. This is the first quantification of the cell‐killing capabilities of the UCNP/TPP composite nanoparticles formulated for photodynamic therapy.