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Ultrasensitive and Selective Detection of a Prognostic Indicator in Early‐Stage Cancer Using Graphene Oxide and Carbon Nanotubes
Author(s) -
Wang Xiaohui,
Wang Chunyan,
Qu Konggang,
Song Yujun,
Ren Jinsong,
Miyoshi Daisuke,
Sugimoto Naoki,
Qu Xiaogang
Publication year - 2010
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.201001118
Cyclin A 2 is a prognostic indicator in early‐stage cancer. However, since most of peptide‐protein bindings do not produce an easily measurable output signal, this severely hinders homogeneous detection of protein using peptide as detection probe. Cyclin A 2 can be detected as low as 0.6 μ M using a well‐known p21 (WAF‐1) consensus senquence for a specific cyclin A 2 binding motif, which is incorporated into a Tb 3+ chelating macrocycle by chemical modification at the N‐terminus of CMB. Herein, a simple, ultra‐sensitive, and selective signal‐on fluorescence assay is developed for detection of a prognostic indicator in early‐stage cancer, cyclin A 2 . Graphene oxide (GO) is even superior to SWNTs for cyclin A 2 detection. The direct detection limit using graphene oxide is 0.5 n M , 10‐fold better than using SWNTs, and 1200‐fold better than the latest reported value of 0.6 μ M using the Tb 3+ chelating macrocycle modified p21 (WAF‐1) peptide.