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Protein Immobilization: Capturing Complex Protein Gradients on Biomimetic Hydrogels for Cell‐Based Assays (Adv. Funct. Mater. 21/2009)
Author(s) -
Cosson Steffen,
Kobel Stefan A.,
Lutolf Matthias P.
Publication year - 2009
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.200990095
Subject(s) - self healing hydrogels , ethylene glycol , materials science , microfluidics , nanotechnology , orthogonality , peg ratio , biophysics , biomimetics , chemical engineering , polymer chemistry , biology , geometry , mathematics , finance , engineering , economics
A versatile microfluidic strategy to rapidly and selectively immobilize gradients of virtually any desired protein on soft poly(ethylene glycol) (PEG) hydrogel surfaces is developed by S. Cosson et al. on page 3411 . The selectivity and orthogonality of the chosen protein immobilization schemes allows for forming parallel and orthogonal overlapping gradients of multiple proteins. This platform can be exploited to perform a wealth of cell‐based assays on biomimetic surfaces.