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Stem Cell Aligned Growth Induced by CeO 2 Nanoparticles in PLGA Scaffolds with Improved Bioactivity for Regenerative Medicine
Author(s) -
Mandoli Corrado,
Pagliari Francesca,
Pagliari Stefania,
Forte Giancarlo,
Di Nardo Paolo,
Licoccia Silvia,
Traversa Enrico
Publication year - 2010
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.200902363
Subject(s) - materials science , plga , ceramic , nanoparticle , scaffold , nanotechnology , mesenchymal stem cell , chemical engineering , biomedical engineering , composite material , medicine , engineering , microbiology and biotechnology , biology
Hybrid 2D polymeric–ceramic biosupports are fabricated by mixing a nanostructured CeO 2 powder with 85:15 poly( D,L ‐lactic‐ co ‐glycolic acid) (PLGA)/dichloromethane solutions at specific concentrations, followed by solvent casting onto pre‐patterned molds. The mold patterning allows the orientation of ceramic nanoparticles into parallel lines within the composite scaffold. The ability of the produced films to host and address cell growth is evaluated after 1, 3, and 6 days of culturing with murine derived cardiac and mesenchymal stem cells (CSCs and MSCs), and compared with PLGA films without ceramics and loaded with nanostructured TiO 2 . Aligned cell growth is observed only for scaffolds that incorporate oriented ceramic nanoparticles, attributed to the nanoceramic ability to modulate the roughness pitch, thus improving cell sensitivity towards the host surface features. Better CSC and MSC proliferative activity is observed for CeO 2 composites with respect to either TiO 2 ‐added or unfilled PLGA films. This evidence may be related to the nanostructured CeO 2 antioxidative properties.