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Conjugated Polymers Combined with a Molecular Beacon for Label‐Free and Self‐Signal‐Amplifying DNA Microarrays
Author(s) -
Lee Kangwon,
Rouillard JeanMarie,
Kim BongGi,
Gulari Erdogan,
Kim Jinsang
Publication year - 2009
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.200901175
Subject(s) - molecular beacon , förster resonance energy transfer , conjugated system , fluorescence , oligonucleotide , covalent bond , combinatorial chemistry , materials science , photochemistry , biosensor , amine gas treating , molecule , polymer , dna , chemistry , organic chemistry , nanotechnology , biochemistry , physics , quantum mechanics
A conjugated polymer (CP) and molecular‐beacon‐based solid‐state DNA sensing system is developed to achieve sensitive, label‐free detection. A novel conjugated poly(oxadiazole) derivative exhibiting amine and thiol functional groups ( POX‐SH ) is developed for unique chemical and photochemical stability and convenient solid‐state on‐chip DNA synthesis. POX‐SH is soluble in most nonpolar organic solvents and exhibits intense blue fluorescence. POX‐SH is covalently immobilized onto a maleimido‐functionalized glass slide by means of its thiol group. Molecular beacons having a fluorescent dye or quencher molecule as the fluorescence resonance energy transfer (FRET) acceptor are synthesized on the immobilized POX‐SH layer through direct on‐chip oligonucleotide synthesis using the amine side chain of POX‐SH . Selective hybridization of the molecular beacon probes with the target DNA sequence opens up the molecular beacon probes and affects the FRET between POX‐SH and the dye or quencher, producing a sensitive and label‐free fluorescence sensory signal. Various molecular design parameters, such as the size of the stem and loop of the molecular beacon, the choice of dye, and the number of quencher molecules are systematically controlled, and their effects on the sensitivity and selectivity are investigated.