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In Situ Bioconjugation: Single Step Approach to Tailored Nanoparticle‐Bioconjugates by Ultrashort Pulsed Laser Ablation
Author(s) -
Petersen Svea,
Barcikowski Stephan
Publication year - 2009
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.200801526
Subject(s) - bioconjugation , materials science , laser ablation synthesis in solution , biomolecule , nanotechnology , nanoparticle , oligonucleotide , laser ablation , in situ , laser , chemistry , dna , laser power scaling , optics , organic chemistry , x ray laser , biochemistry , physics
A single step approach to tailored nanoparticle‐bioconjugates, enabling the generation of gold nanoparticles by laser ablation and their in situ conjugation with any biomolecule bearing an electron donating function (e.g., thiolated oligonucleotides) is established. The integrity of oligonucleotides after conjugation and the stability of bioconjugates in physiological media are investigated. Their size is tailorable via process parameters. This rapid and universal method may provide biochemists with various nanoparticle‐bioconjugates for screening the often unpredictable structure–function relationship.