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pH‐Responsive Flower‐Type Micelles Formed by a Biotinylated Poly(2‐vinylpyridine)‐ block ‐poly(ethylene oxide)‐ block ‐poly( ε ‐caprolactone) Triblock Copolymer
Author(s) -
Van Butsele Kathy,
Cajot Sébastien,
Van Vlierberghe Sandra,
Dubruel Peter,
Passirani Catherine,
Benoit JeanPierre,
Jérôme Robert,
Jérôme Christine
Publication year - 2009
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.200801117
Subject(s) - copolymer , micelle , dynamic light scattering , biotinylation , ethylene oxide , polymerization , caprolactone , polymer chemistry , streptavidin , materials science , chemistry , aqueous solution , chemical engineering , nanoparticle , organic chemistry , polymer , nanotechnology , biotin , engineering , biochemistry
In the present work, a method is proposed to assemble pH‐responsive, flower‐like micelles that can expose a targeting unit at their periphery upon a decrease in pH. The micelles are composed of a novel biotinylated triblock copolymer of poly( ε ε ‐caprolactone)‐ block ‐poly(ethylene oxide)‐ block ‐poly(2‐vinylpyridine) (PCL‐ b ‐PEO‐ b ‐P2VP) and the non‐biotinylated analogue. The block copolymers are synthesized by sequential anionic and ring‐opening polymerization. The pH‐dependent micellization behaviour in aqueous solution of the triblock copolymers developed is studied using dynamic light scattering, zeta potential, transmission electron microscopy (TEM), and fluorimetric measurements. The shielding of the biotin at neutral pH and their availability at the micelle surface upon protonation is established by TEM and surface plasmon resonance with avidin and streptavidin‐coated gold surfaces. The preliminary stealthy behavior of these pH‐responsive micelles is examined using the complement activation (CH50) test.