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Self‐assembly of Protein Nanoarrays on Block Copolymer Templates
Author(s) -
Lau K. H. Aaron,
Bang Joona,
Kim Dong Ha,
Knoll Wolfgang
Publication year - 2008
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.200800487
Subject(s) - biomolecule , template , materials science , nanotechnology , nanoscopic scale , copolymer , lithography , polystyrene , nanostructure , self assembly , bovine serum albumin , protein adsorption , polymer , chemistry , biochemistry , optoelectronics , composite material
There is considerable interest in developing functional protein arrays on the nanoscale for high‐throughput protein‐based array technology, and for the study of biomolecular and cell interactions at the physical scale of the biomolecules. To these ends, self‐assembly based techniques may be desirable for the nanopatterning of proteins on large sample areas without the use of lithography equipment. We present a fast, general approach for patterning proteins (and potentially other biomolecules) on the nanoscale, which takes advantage of the ability of block copolymers to self‐assemble into ordered surface nanopatterns with defined chemical heterogeneity. We demonstrate nanoarrays of immunoglobulin and bovine serum albumin on polystyrene‐block‐poly(methyl methacrylate) templates, and illustrate the applicability of our technique through immunoassays and DNA sensing performed on the protein nanoarrays. Furthermore, we show that the pattern formation mechanism is a nanoscale effect originating from a combination of fluid flow forces and geometric restrictions templated by an underlying nanopattern with a difference in protein adsorption behavior on adjacent, chemically distinct surfaces. This understanding may provide a framework for extending the patterning approach to other proteins and material systems.