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Gold Nanoparticle–Colloidal Carbon Nanosphere Hybrid Material: Preparation, Characterization, and Application for an Amplified Electrochemical Immunoassay
Author(s) -
Cui Rongjing,
Liu Chang,
Shen Jianming,
Gao Di,
Zhu JunJie,
Chen HongYuan
Publication year - 2008
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.200701340
Subject(s) - materials science , colloidal gold , biosensor , amperometry , biomolecule , nanotechnology , detection limit , dispersity , horseradish peroxidase , carbon nanotube , nanoparticle , biocompatibility , carbon fibers , electrode , chemical engineering , electrochemistry , chromatography , composite number , chemistry , organic chemistry , polymer chemistry , composite material , engineering , metallurgy , enzyme
A rapid microwave‐hydrothermal method has been developed to prepare monodisperse colloidal carbon nanospheres from glucose solution, and gold nanoparticles (AuNPs) are successfully assembled on the surface of the colloidal carbon nanospheres by a self‐assembly approach. The resulting AuNP/colloidal carbon nanosphere hybrid material (AuNP/C) has been characterized and is expected to offer a promising template for biomolecule immobilization and biosensor fabrication because of its satisfactory chemical stability and the good biocompatibility of AuNPs. Herein, as an example, it is demonstrated that the as‐prepared AuNP/C hybrid material can be conjugated with horseradish peroxidase‐labeled antibody (HRP‐Ab 2 ) to fabricate HRP‐Ab 2 ‐AuNP/C bioconjugates, which can then be used as a label for the sensitive detection of protein. The amperometric immunosensor fabricated on a carbon nanotube‐modified glass carbon electrode was very effective for antibody immobilization. The approach provided a linear response range between 0.01 and 250 ng mL −1 with a detection limit of 5.6 pg mL −1 . The developed assay method was versatile, offered enhanced performances, and could be easily extended to other protein detection as well as DNA analysis.