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Functionalization of Cubic I a 3d Mesoporous Silica for Immobilization of Penicillin G Acylase
Author(s) -
Lü Y.,
Lu G.,
Wang Y.,
Guo Y.,
Guo Y.,
Zhang Z.,
Wang Y.,
Liu X.
Publication year - 2007
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.200600505
Subject(s) - materials science , mesoporous material , fourier transform infrared spectroscopy , surface modification , adsorption , mesoporous silica , hydrolysis , chemical engineering , covalent bond , nuclear chemistry , polymer chemistry , organic chemistry , catalysis , chemistry , engineering
Functionalized cubic Ia 3 d mesoporous silica (FCIMS) with glycidoxypropyl groups was prepared and characterized by powder XRD, N 2 adsorption, TGA, FT‐IR spectroscopy, solid state 13 C NMR spectroscopy and TEM, and studied as the support for the immobilization of Penicillin G acylase (PGA). The results show that the glycidoxypropyl groups have been chemically bonded to the silicon atoms on the surface of cubic Ia 3 d mesoporous silica (CIMS). The FCIMS materials possess the mesoporous structure with pore diameter of 8 nm which is ∼ 1 nm less than that of CIMS (9 nm). The influence of the amount of glycidoxypropyl groups on the initial specific activity and operational stability of PGA immobilized on FCIMS were examined for the hydrolysis of penicillin G potassium salt. The results show that PGA has been successfully immobilized covalently on the FCIMS materials, and the initial specific activity of PGA/FCIMS is 835 IU g –1 and PGA/CIMS is 624 IU g –1 . After repeated use for 10 times, PGA/FCIMS retains 72 % of its initial specific activity and PGA/CIMS retains only 58 %.