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Selective Immobilization of Protein Clusters on Polymeric Nanocraters
Author(s) -
Valsesia A.,
Colpo P.,
Meziani T.,
Bretagnol F.,
Lejeune M.,
Rossi F.,
Bouma A.,
GarciaParajo M.
Publication year - 2006
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.200500653
Subject(s) - materials science , covalent bond , acrylic acid , ethylene glycol , fluorescence microscope , plasma enhanced chemical vapor deposition , carboxylic acid , fluorescence , chemical force microscopy , chemical engineering , nanotechnology , chemical vapor deposition , confocal microscopy , polymer , polymer chemistry , atomic force microscopy , copolymer , organic chemistry , chemistry , composite material , optics , non contact atomic force microscopy , physics , kelvin probe force microscope , engineering
A method for fabricating chemically nanopatterned surfaces based on a combination of colloidal lithography and plasma‐ enhanced chemical vapor deposition (PECVD) is presented. This method can be applied for the creation of different nanopatterns, and it is in principle not limited in patterning resolution. Nanocraters of poly(acrylic acid) (carboxylic moieties) surrounded by a matrix of poly(ethylene glycol) are fabricated. Chemical force microscopy demonstrates that the process is able to produce the expected surface chemical contrast. Finally, the carboxylic groups of the craters are activated in order to induce the covalent binding of fluorescent‐labeled proteins. Fluorescence investigation using scanning confocal microscopy shows that the proteins are preferentially attached inside the functional craters.