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Biomineralized Polysaccharide Capsules for Encapsulation, Organization, and Delivery of Human Cell Types and Growth Factors
Author(s) -
Green D. W.,
Leveque I.,
Walsh D.,
Howard D.,
Yang X.,
Partridge K.,
Mann S.,
Oreffo R. O. C.
Publication year - 2005
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/adfm.200400322
Subject(s) - mesenchymal stem cell , materials science , bone morphogenetic protein 2 , chitosan , biophysics , mineralization (soil science) , cell encapsulation , tissue engineering , polysaccharide , cell , in vivo , nanotechnology , microbiology and biotechnology , biomedical engineering , in vitro , chemistry , biochemistry , biology , organic chemistry , nitrogen , medicine
The construction of biomimetic microenvironments with specific chemical and physical cues for the organization and modulation of a variety of cell populations is of key importance in tissue engineering. We show that a range of human cell types, including promyoblasts, chondrocytes, adipocytes, adenovirally transduced osteoprogenitors, immunoselected mesenchymal stem cells, and the osteogenic factor, rhBMP‐2 (BMP: bone morphogenic protein), can be successfully encapsulated within mineralized polysaccharide capsules without loss of function in vivo. By controlling the extent of mineralization within the alginate/chitosan shell membrane, degradation of the shell wall and release of cells or rhBMP‐2 into the surrounding medium can be regulated. In addition, we describe for the first time the ability to generate bead‐in‐bead capsules consisting of spatially separated cell populations and temporally separated biomolecule release, entrapped within alginate/chitosan shells of variable thickness, mineralization, and stability. Such materials offer significant potential as multifunctional scaffolds and delivery vehicles in tissue regeneration of hard and soft tissues.