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Large‐Scale Fabrication of 3D Scaffold‐Based Patterns of Microparticles and Breast Cancer Cells using Reusable Acoustofluidic Device
Author(s) -
Nguyen Tan Dai,
Tran Van-Thai,
Pudasaini Sanam,
Gautam Archana,
Lee Jia Min,
Fu Yong Qing,
Du Hejun
Publication year - 2021
Publication title -
advanced engineering materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.938
H-Index - 114
eISSN - 1527-2648
pISSN - 1438-1656
DOI - 10.1002/adem.202001377
Subject(s) - scaffold , materials science , fabrication , 3d printed , tissue engineering , biocompatibility , reusability , biomedical engineering , nanotechnology , 3d printing , computer science , composite material , engineering , software , medicine , alternative medicine , pathology , metallurgy , programming language
Spatial distribution of biological cells plays a key role in tissue engineering for reconstituting the cellular microenvironment, and recently, acoustofluidics are explored as a viable tool for controlling structures in tissue fabrication because of its good biocompatibility, low‐power consumption, automation capability, nature of non‐invasive, and non‐contact. Herein, a reusable acoustofluidic device is developed using surface acoustic waves for manipulating microparticles/cells to form a 3D matrix pattern inside a scaffold‐based hydrogel contained in a millimetric chamber. The 3D patterned and polymerized hydrogel construct can be easily and safely removed from the chamber using a proposed lifting technique, which prevent any physical damages or contaminations and promote the reusability of the chamber. The generated 3D patterns of microparticles and cells are numerically studied using a finite‐element method, which is well validated by the experimental results. The proposed acoustofluidic device is a useful tool for in vitro engineering 3D scaffold‐based artificial tissues for drug and toxicity testing and building organs‐on‐chip applications.