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Influence of Silicification on the Structural and Biological Properties of Buffer‐Mediated Collagen Hydrogels
Author(s) -
Quignard Sandrine,
Copello Guillermo J.,
Aimé Carole,
Bataille Isabelle,
Hélary Christophe,
Desimone Martin F.,
Coradin Thibaud
Publication year - 2012
Publication title -
advanced engineering materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.938
H-Index - 114
eISSN - 1527-2648
pISSN - 1438-1656
DOI - 10.1002/adem.201180063
Subject(s) - chemical engineering , composite number , rheology , colloid , self healing hydrogels , materials science , buffer (optical fiber) , homogeneous , nanoparticle , silicate , particle (ecology) , chemistry , biophysics , nanotechnology , polymer chemistry , composite material , oceanography , physics , geology , engineering , thermodynamics , telecommunications , biology , computer science
Abstract A buffer‐mediated gelation route for collagen hydrogels that allows the formation of homogeneous composite and hybrid materials with various silica sources (i.e., colloidal silica and soluble silicates) at high concentration (up to 25 × 10 −3 M ) is described. Most significant improvement in rheological properties and proliferation of primary adult human dermal fibroblasts was obtained for the silicate‐based hybrid materials. A similar trend was observed in composite materials incorporating 14 nm SiO 2 nanoparticles, although to a much lesser extent, whereas larger colloids (80 and 390 nm) did not significantly impact mechanical stability and cell behavior. Modification of 80 nm particles surface with amine groups weakens the collagen‐mineral interface, resulting in the decrease of material stability and leading to particle aggregation during the course of cell proliferation experiments.