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Unconventional Multi‐Scale Patterning of Titanium Dioxide: A New Tool for the Investigation of Cell–Topography Interactions
Author(s) -
Biscarini Fabio,
Bianchi Michele,
Chelli Beatrice,
Valle Francesco,
Dionigi Chiara,
Bystrenova Eva,
Greco Pierpaolo
Publication year - 2012
Publication title -
advanced engineering materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.938
H-Index - 114
eISSN - 1527-2648
pISSN - 1438-1656
DOI - 10.1002/adem.201180061
Subject(s) - materials science , porosity , adhesion , nanotechnology , titanium dioxide , cell adhesion , titanium , micrometer , substrate (aquarium) , nanometre , texture (cosmology) , biocompatible material , polystyrene , silicon dioxide , biomedical engineering , composite material , polymer , optics , medicine , oceanography , physics , image (mathematics) , artificial intelligence , computer science , metallurgy , geology
Titanium dioxide (TiO 2 ) is a biocompatible material with important applications in the field of regenerative medicine. Here we show that a multi‐scale hierarchical architecture of TiO 2 , realized with sub‐micrometer polystyrene beads as templating agent patterned by “micromolding in capillaries” (MIMICs), is a viable functional tool for the systematic investigation of cell behavior with respect to a complex topographic texture of the substrate. TiO 2 stripes of different width and interconnected porosity whose size ranges from a few hundred to a few tens nanometer, are obtained after thermal treatment of the precursors with concurrent removal of the templating agent. The adhesion and proliferation of two human secondary neural cell lines, i.e., 1321N1 astrocytoma and SH‐SY5Y neuroblastoma, on the patterns is statistically assessed with respect to the TiO 2 stripe width and porosity. Our results show that cells have a strong preference for TiO 2 patterns with respect to glass, the proliferation rate is not affected by cell porosity whereas adhesion is although ligthly, whereas the response of cell density to stripe width is very different in astocytoma cells with respect to neuroblastoma cells.