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Biocompatible Smart Microcapsules Based on Chitosan‐Poly(vinyl alcohol) Copolymers for Cultivation of Animal Cells
Author(s) -
ZaytsevaZotova Daria,
Balysheva Vera,
Tsoy Anna,
Drozdova Maria,
Akopova Tatiana,
Vladimirov Leonid,
Chevalot Isabelle,
Marc Annie,
Goergen JeanLouis,
Markvicheva Elena
Publication year - 2011
Publication title -
advanced engineering materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.938
H-Index - 114
eISSN - 1527-2648
pISSN - 1438-1656
DOI - 10.1002/adem.201180014
Subject(s) - vinyl alcohol , chitosan , in vitro , copolymer , materials science , biocompatible material , cell culture , cell growth , monoclonal antibody , drug delivery , polymer chemistry , chemistry , nanotechnology , antibody , biochemistry , biomedical engineering , polymer , biology , immunology , medicine , composite material , genetics
In this study, two novel chitosan‐graft‐poly(vinyl alcohol) copolymers are synthesized and used as water‐soluble at physiological conditions polycations for preparation of smart microcapsules. The microcapsules provide growth and proliferation of eight mammalian cell lines, including hybridoma and tumor cells, at long‐term cell cultivation in vitro. The microcapsules are stable in cell culture medium but can be dissolved by changing pH value of the medium (up to 8.0–8.2), thus making possible a simple release of the entrapped cells. Monoclonal antibody production by encapsulated hybridoma cells is demonstrated. Cultivation of tumor cells within the microcapsules allows the formation of 3D multicellular spheroids, which can be proposed as an in vitro model for anticancer drug screening.