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Porous Poly(ε‐caprolactone) Nerve Guide Filled with Porous Gelatin Matrix for Nerve Tissue Engineering
Author(s) -
TondaTuro Chiara,
Audisio Chiara,
Gnavi Sara,
Chiono Valeria,
Gentile Piergiorgio,
Raimondo Stefania,
Geuna Stefano,
Perroteau Isabelle,
Ciardelli Gianluca
Publication year - 2011
Publication title -
advanced engineering materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.938
H-Index - 114
eISSN - 1527-2648
pISSN - 1438-1656
DOI - 10.1002/adem.201080099
Subject(s) - materials science , gelatin , porosity , genipin , microporous material , permeation , composite material , membrane , chemical engineering , matrix (chemical analysis) , polycaprolactone , tissue engineering , adhesion , biomedical engineering , polymer , chitosan , chemistry , organic chemistry , medicine , biochemistry , engineering
The preparation and characterization of PCL guides filled with a porous matrix of a freeze‐dried, genipin‐crosslinked gelatin sponge (GL/GP) is described. Porous guides are obtained by dip coating a rotating mandrel with a PCL/PEO blend solution followed by PEO solvent extraction. PCL/PEO 60/40 is selected as the optimal composition to obtain model porous membranes with suitable morphological properties to ensure nutrient permeation and moderate stiffness. GL/GP sponges show increased mechanical strength and water stability, compared to uncrosslinked GL sponges. GL/GP in the form of films, sponges and internal fillers support the in vitro adhesion and proliferation of Schwann like cells.