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Attomolar Electrochemical Detection of DNA Hybridization Based on Enhanced Latex/Gold Nanoparticles
Author(s) -
Pinijsuwan Suttiporn,
Rijiravanich Patsamon,
Somasundrum Mithran,
Surareungchai Werasak
Publication year - 2010
Publication title -
advanced engineering materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.938
H-Index - 114
eISSN - 1527-2648
pISSN - 1438-1656
DOI - 10.1002/adem.201080039
Subject(s) - materials science , anodic stripping voltammetry , detection limit , colloidal gold , differential pulse voltammetry , electrochemistry , stripping (fiber) , nanoparticle , voltammetry , dna–dna hybridization , analytical chemistry (journal) , anode , electrode , micrometer , autocatalysis , layer (electronics) , cyclic voltammetry , nanotechnology , dna , chromatography , chemistry , composite material , organic chemistry , optics , biochemistry , physics , catalysis
We report a highly sensitive method for detecting DNA hybridization by anodic‐stripping voltammetry, using assemblies of AuNPs as electrochemical labels. The assemblies are made by layer‐by‐layer modification of sub‐micrometer latex spheres, followed by the uptake of the negatively charged AuNPs by ion exchange. The Au content can be considerably enhanced by autocatalytic reduction. Under the optimized conditions of enhancement, using differential pulse voltammetry for the stripping, 30 mer targets common to five strains of E. coli could be calibrated across the range 10 × 10 −18 M to 100 × 10 −18 M with a detection limit of 20 × 10 −18 M , which corresponds to ≈240 DNA hybridizations.