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Phase Contrast X‐Ray Tomographic Microscopy for Biological and Materials Science Applications
Author(s) -
McDonald Samuel A.,
Marone Federica,
Hintermüller Christoph,
Mikuljan Gordan,
David Christian,
Stampai Marco
Publication year - 2011
Publication title -
advanced engineering materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.938
H-Index - 114
eISSN - 1527-2648
pISSN - 1438-1656
DOI - 10.1002/adem.201000219
Subject(s) - beamline , tomographic reconstruction , optics , materials science , phase contrast imaging , microscopy , phase retrieval , tomography , resolution (logic) , phase (matter) , interferometry , grating , differential interference contrast microscopy , phase contrast microscopy , physics , computer science , artificial intelligence , fourier transform , beam (structure) , quantum mechanics
The application of two approaches for high‐throughput, high‐resolution X‐ray phase contrast tomographic imaging being used at the tomographic microscopy and coherent radiology experiments (TOMCAT) beamline of the SLS is discussed and illustrated. Differential phase contrast (DPC) imaging, using a grating interferometer and a phase‐stepping technique, is integrated into the beamline environment at TOMCAT in terms of the fast acquisition and reconstruction of data and the availability to scan samples within an aqueous environment. A second phase contrast method is a modified transfer of intensity approach that can yield the 3D distribution of the decrement of the refractive index of a weakly absorbing object from a single tomographic dataset. The two methods are complementary to one another: the DPC method is characterised by a higher sensitivity and by moderate resolution with larger samples; the modified transfer of intensity approach is particularly suited for small specimens when high resolution (around 1 µm) is required. Both are being applied to investigations in the biological and materials science fields.