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Synthesis and Characterization of Thrombin Conjugated γ‐Fe 2 O 3 Magnetic Nanoparticles for Hemostasis
Author(s) -
Ziv Ofra,
LublinTennenbaum Tammy,
Margel Shlomo
Publication year - 2009
Publication title -
advanced engineering materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.938
H-Index - 114
eISSN - 1527-2648
pISSN - 1438-1656
DOI - 10.1002/adem.200990036
Subject(s) - thrombin , hemostasis , discovery and development of direct thrombin inhibitors , nanoparticle , materials science , biophysics , combinatorial chemistry , chemistry , nanotechnology , platelet , immunology , biology , medicine , surgery
Thrombin is the final protease produced in the clotting pathways. Thrombin has been used in the clinic more than six decades for topical hemostasis and wound management. In human plasma the half‐life of thrombin is shorter than 15 seconds due to close control by inhibitors. In order to stabilize thrombin, this enzyme was conjugated covalently and physically to γ‐Fe 2 O 3 magnetic nanoparticles. The physical conjugation was accomplished through adsorption of thrombin to BSA coating on the nanoparticles. The coagulant activity of the covalently bound thrombin was significantly lower than that of the physically adsorbed thrombin. Leakage of the physically bound thrombin into PBS containing 4% HSA was negligible. The physical conjugation of thrombin onto the nanoparticles stabilized the thrombin against its major inhibitor antithrombin III and improved its storage stability. At optimal CaCl 2 concentration, the clotting time by the bound thrombin is shorter than that of the free enzyme. This novel conjugated thrombin may be an efficient candidate for topical hemostasis and wound healing.