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Hydroxyapatite/SiO 2 Composites via Freeze Casting for Bone Tissue Engineering
Author(s) -
Blindow Silke,
Pulkin Maxim,
Koch Dietmar,
Grathwohl Georg,
Rezwan Kurosch
Publication year - 2009
Publication title -
advanced engineering materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.938
H-Index - 114
eISSN - 1527-2648
pISSN - 1438-1656
DOI - 10.1002/adem.200900208
Subject(s) - materials science , sintering , shrinkage , porosity , ceramic , composite material , casting , fabrication , surface roughness , adhesion , osteocalcin , alkaline phosphatase , medicine , biochemistry , chemistry , alternative medicine , pathology , enzyme
Freeze casting is a fabrication method that allows producing near‐net‐shaped ceramics with variable porosity. Hydroxyapatite (HA) was modified by the addition of different amounts of SiO 2 nanoparticles during freeze cast preparation. The addition of SiO 2 introduced a partial phase transformation of HA to β ‐tricalcium phosphate and improved the form stability due to less shrinkage after sintering. The impact of surface roughness of pure HA ceramics and the influence of SiO 2 introduction during freeze casting on adhesion, proliferation, and differentiation of human osteoblast‐like cells (MG‐63) was investigated. While both cell attachment and proliferation of smooth pressed HA was significantly enhanced compared to rough freeze cast HA, the addition of SiO 2 improved the cell numbers of the latter. The expression of cell differentiation markers osteocalcin and collagen I was found to be supported by rough surfaces ( R a = 5–6 µm) in particular on ceramics containing SiO 2