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Mass Fabrication of Small Cell Spheroids by Using Micro‐patterned Tissue Culture Plate
Author(s) -
Iwasaki Akinari,
Matsumoto Takuya,
Tazaki Go,
Tsuruta Hitoshi,
Egusa Hiroshi,
Miyajima Hiroyuki,
Sohmura Taiji
Publication year - 2009
Publication title -
advanced engineering materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.938
H-Index - 114
eISSN - 1527-2648
pISSN - 1438-1656
DOI - 10.1002/adem.200800392
Subject(s) - spheroid , materials science , fabrication , adhesion , pipette , cell adhesion , nanotechnology , cell , biomedical engineering , biophysics , cell culture , composite material , chemistry , biology , pathology , medicine , biochemistry , genetics , alternative medicine
A newly designed micro‐patterned chamber was utilized to fabricate cell spheroids with a constant size (<200 μm) and cell number. By applying cytochalasin D as a chemical to control cell adhesion and aggregation, thousands of aggregated cells were formed in each patterned chamber. Importantly, the formed cell spheroids were collected by a simple pipetting process without using proteinase.