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Fabrication of Galactosylated Polyethylenimine and Plasmid DNA Multilayers on poly ( D , L ‐lactic acid) Films for in situ Targeted Gene Transfection
Author(s) -
Hu Yan,
Cai Kaiyong,
Luo Zhong,
Chen Chong,
Dong Haide,
Hao Jianyuan,
Yang Li,
Deng Linhong
Publication year - 2009
Publication title -
advanced engineering materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.938
H-Index - 114
eISSN - 1527-2648
pISSN - 1438-1656
DOI - 10.1002/adem.200800342
Subject(s) - polyethylenimine , transfection , in situ , gene delivery , materials science , microbiology and biotechnology , plasmid , tissue engineering , dna , gene , biophysics , biology , chemistry , biochemistry , genetics , organic chemistry
This study presents surface‐mediated targeted in situ gene delivery from gene‐tagged poly( D , L ‐lactic acid) (PDLLA) films, which were fabricated via a layer‐by‐layer (LbL) assembly technique with galactosylated polyethylenimine (GP) and plasmid DNA (pDNA, pSV‐β‐galactosidase). A linear growth of GP/pDNA multilayered films was observed. The pDNA was continuously released from multilayered films for over 32 h. The multilayered structure degraded and simultaneously formed GP/pDNA complexes in situ when exposing to a physiological environment. The pDNA was well protected by GP against DNase I digestion within formed GP/pDNA complexes. Our results demonstrated that GP contributes to receptor‐mediated targeting for cell uptake and in situ gene transfection. The results reported here are potentially important for gene therapy, surface engineering of biomaterials, tissue engineering and implant technology.