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The PD‐1 Interactome (Adv. Biology 9/2021)
Author(s) -
Wang Qi,
Bardhan Kankana,
Boussiotis Vassiliki A.,
Patsoukis Nikolaos
Publication year - 2021
Publication title -
advanced biology
Language(s) - English
Resource type - Journals
ISSN - 2701-0198
DOI - 10.1002/adbi.202170093
Subject(s) - bimolecular fluorescence complementation , dapi , microbiology and biotechnology , kinase , hek 293 cells , fyn , chemistry , biology , biophysics , biochemistry , proto oncogene tyrosine protein kinase src , apoptosis , receptor , yeast
Programmed Death‐1 In article 2100758, Boussiotis, Patsoukis, Wang, and Bardhan show that Venus‐based biomolecular fluorescence complementation detects Programmed Death‐1 (PD‐1) dimerization by confocal microscopy. HEK‐293 cells are transfected with PD‐1‐VenusYFP‐N‐terminus and PD‐1‐VenusYFP‐C‐terminus together with SHP‐2 and either kinase active (top) or kinase inactive (bottom) Fyn. YFP signal (yellow) from VenusYFP structural complementation, indicating PD‐1 dimerization at the plasma membrane, is generated only in the presence of kinase active Fyn (red: Rhodamine Phalloidin; blue: DAPI nuclear staining).