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Wound Healing: Blue Light‐Operated CRISPR/Cas13b‐Mediated mRNA Knockdown (Lockdown) (Adv. Biology 5/2021)
Author(s) -
Blomeier Tim,
Fischbach Patrick,
Koch LeonieAlexa,
Andres Jennifer,
Miñambres Miguel,
Beyer Hannes Michael,
Zurbriggen Matias Daniel
Publication year - 2021
Publication title -
advanced biology
Language(s) - English
Resource type - Journals
ISSN - 2701-0198
DOI - 10.1002/adbi.202170051
Subject(s) - gene knockdown , optogenetics , crispr , messenger rna , downregulation and upregulation , blue light , biology , microbiology and biotechnology , rna interference , rna , gene , genetics , optoelectronics , physics , neuroscience
In article number 2000307, Tim Blomeier and co‐workers describe the Blue L ight‐ O perated C RISPR/Cas13b‐mediated mRNA K nock down . “Lockdown” introduces optical control of RNA levels utilizing a blue light‐dependent switch to induce expression of CRISPR/Cas13b, which mediates sequence‐specific knockdown of exo‐ and endogenous mRNA. Further, they combined the tool with other optogenetic switches for the efficient downregulation of target proteins in mammalian cells.