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Protein Direct Interactome: Identification of Protein Direct Interactome with Genetic Code Expansion and Search Engine OpenUaa (Adv. Biology 3/2021)
Author(s) -
Liu Chao,
Wu Ting,
Shu Xin,
Li ShangTong,
Wang Daniel R.,
Wang Nanxi,
Zhou Rong,
Yang Hao,
Jiang Hong,
Hendriks Ivo A.,
Gong Pengyun,
Zhang Long,
Nielsen Michael L.,
Li Kui,
Wang Lei,
Yang Bing
Publication year - 2021
Publication title -
advanced biology
Language(s) - English
Resource type - Journals
ISSN - 2701-0198
DOI - 10.1002/adbi.202170032
Subject(s) - interactome , identification (biology) , computational biology , genetic code , chemistry , amino acid , biology , biochemistry , gene , botany
Genetically encoded chemical crosslinking (GECX) via bioreactive unnatural amino acid (Uaa) enables the capture of weak and transient protein‐protein interactions in live cells for identification with mass spectrometry. In article number 2000308, Chao Liu, Shang‐Tong Li, Kui Li, Lei Wang, Bing Yang, and co‐workers develop a new software OpenUaa to analyze Uaa‐crosslinked peptides, which uniquely considers all fragment ions for high sensitivity and identify direct protein interactome with the GECX‐OpenUaa strategy.